A2M possesses anti-bacterial functions by recruiting and enhancing phagocytosis through GRP78 in an echinoderm.

Alpha-2-macroglobulin (A2M) is an extracellular macromolecule mainly known for its role as a broad-spectrum protease inhibitor in mammals. However, the immune recognition and regulation mechanisms of A2M in invertebrates are still not well investigated. In the current study, the role of sea cucumber Apostichopus japonicus A2M in the regulation of innate immune responses was explored. We found that AjA2M promotes phagocytosis of Vibrio splendidus in coelomocytes of sea cucumber. Then two major functional structural domains of AjA2M, the thioester domain (TED) and the receptor-binding structural domain (RBD) were cloned. It was found that the AjA2M-TED binds to pathogens while causing Vibrio splendidus aggregation; the AjA2M-RBD interacts with the Glucose Regulated Protein 78 (AjGRP78), subsequently AjGRP78 accelerates the degradation of Vibrio splendidus in lysosomes by facilitating polymerisation and rearrangement of the cytoskeleton. Collectively, the findings together suggest that A2M-GRP78 axis mediates immune signaling pathway of phagocytosis and AjA2M has been characterized to play an essential crucial role in antibacterial immune responses of invertebrates.

GRIM19 deficiency aggravates metabolic disorder and ovarian dysfunction in PCOS.

CONTEXT: Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders in women. Retinoid-interferon-induced mortality 19 (GRIM19) is a functional component of mitochondrial complex I that plays a role in cellular energy metabolism. However, the role of GRIM19 in the pathogenesis of PCOS is still unclear. OBJECTIVE: To investigate the role of GRIM19 in the pathogenesis of PCOS. DESIGN: We first measured the expression of GRIM19 in human granulosa cells (hGCs) from patients with and without PCOS (n = 16 per group), and then established a PCOS mouse model with WT and Grim19+/- mice for in vivo experiments. Glucose uptake-related genes RAC1 and GLUT4 and energy metabolism levels in KGN cells were examined in vitro by knocking down GRIM19 in the cell lines. Additionally, ovulation-related genes such as p-ERK1/2, HAS2, and PTX3 were also studied to determine their expression levels. RESULTS: GRIM19 expression was reduced in hGCs of PCOS patients, which was negatively correlated with BMI and serum testosterone level. Grim19+/- mice with PCOS exhibited a markedly anovulatory phenotype and disturbed glycolipid metabolism. In vitro experiments, GRIM19 deficiency inhibited the RAC1/GLUT4 pathway, reducing insulin-stimulated glucose uptake in KGN cells. Moreover, GRIM19 deficiency induced mitochondrial dysfunction, defective glucose metabolism, and apoptosis. In addition, GRIM19 deficiency suppressed the expression of ovulation-related genes in KGN cells, which was regulated by dihydrotestosterone mediated androgen receptor. CONCLUSIONS: GRIM19 deficiency may mediate ovulation and glucose metabolism disorders in PCOS patients. Our results suggest that GRIM19 may be a new target for diagnosis and treatment.

N-acetylcysteine alleviated tris(2-chloroisopropyl) phosphate-induced sperm motility decline and functional dysfunction in mice through reversing oxidative stress and DNA damage.

The decline in male fertility caused by environmental pollutants has attracted worldwide attention nowadays. Tris(2-chloroisopropyl) phosphate (TCPP) is a chlorine-containing organophosphorus flame retardant applied in many consumer products and has multiple side effects on health. However, whether TCPP impairs spermatogenesis remains unclear. In this study, we found that TCPP reduced the sperm motility and blastocyst formation, inhibited proliferation and induced apoptosis in mice testes and spermatocyte cell line GC-2. Moreover, TCPP induced imbalance of oxidant and anti-oxidant, DNA damage and mitochondrial dysfunction, thus induced abnormal spermatogenesis. In this process, p53 signaling pathway was activated and N-acetylcysteine treatment partially alleviated the side effects of TCPP, including decrease of sperm motility, activation of p53 signaling pathway and DNA damage. Finally, our study verified that TCPP elevated reactive oxygen species (ROS), decreased mitochondrial membrane potential and induced apoptosis in human semen samples. Overall, ROS mediated TCPP-induced germ cell proliferation inhibition and apoptosis, which finally led to the decline of sperm motility.

Cresyl Diphenyl Phosphate exposure induces reproductive functional defects in men and male mice.

Cresyl Diphenyl Phosphate (CDP), as a novel organophosphate esters (OPEs), achieves widely used and exposed in multiple industries. However, its male reproductive toxicity and underlying mechanism remains unclear. In vivo, male mice were gavaged with CDP (0, 4, 20, or 100 mg/kg/d) for 8 weeks. And we treated TM3, TM4 and GC-2 cells with 0, 10, 25, and 50 µM CDP for 24 h to detect its reproductive toxicity effect in vitro. In our study, we revealed that CDP inhibited proliferation and induced apoptosis in mice testis and GC-2 cells, thereby leading to the decreased sperm quality. In mechanism, CDP trigger the oxidative stress and ROS production, thus partially causing DNA damage and cell apoptosis. Moreover, CDP exposure causes injury to Ledyig cells and Sertoli cells, thus disturbing the testicular microenvironment and inhibiting spermatogonia proliferation. In conclusion, this research reveals multiple adverse impacts of CDP on the male reproductive system and calls for further study of the toxicological effects of CDP on human health.

Identification and validation of senescence-related genes in polycystic ovary syndrome.

BACKGROUND: Polycystic ovary syndrome (PCOS) is an exceedingly intractable issue affecting female endocrine and reproductive health. However, the etiology and intricate pathological mechanisms of PCOS remain unclear. Nowadays, aging was found to share multiple common pathological mechanisms with PCOS, which causes probing into the pathogenesis of PCOS from senescence. However, no bioinformatics analyses have specifically focused on connection between PCOS and ovarian aging. METHODS: Differentially expressed aging-related genes in PCOS were identified and then analyzed using function enrichment method. Hub genes were determined based on multiple algorithms, and expression validation of hub genes was performed in both datasets and experiments (human granulosa-like tumor cell line, KGN; human Granulosa Cell, hGCs). Finally, a transcription factor-miRNA-gene network of hub genes was constructed. RESULTS: Here, we identified 73 aging-related differential expression genes (ARDEGs) by intersecting DEGs in PCOS and senescence-related gene set. Furthermore, we performed biological functions and potential pathways of ARDEGs and potential hub genes were also screened by multiple algorithms. From the perspective of immune dysfunction, we analyzed the correlation between PCOS and immune cells. Finally, TF-miRNA-gene networks were constructed. Finally, TF-miRNA-gene networks were constructed. CONCLUSIONS: Our work aimed to elucidate the relation between PCOS and cellular senescence based on bioinformatics strategy, deepening the understanding of mechanisms and to seek for novel therapy strategies for improving reproductive lifespan and female health. Exploring the potential molecular mechanism of cell aging in PCOS is expected to bring a new breakthrough for PCOS diagnosis and therapy strategies. And this, might deepen our understanding about intricate mechanisms of ovarian aging.

A dataset of micro biodiversity in benthic sediment at a global scale.

Microorganisms, occupying the largest biomass in deep sea, play essential roles in deep-sea ecosystem. It is believed that the microbes in deep-sea sediments are more representative of deep-sea microbial communities, the microbial composition of which is seldom affected by ocean currents. However, the community of benthic microbes on a global scale has not been adequately explored. Herein, we build a comprehensive global dataset determined by 16S rRNA gene sequencing to characterize the biodiversity of microorganisms in benthic sediment. The dataset comprised 212 records from 106 sites, included sequencing of bacteria and archaea for each site and yielded 4,766,502 and 1,562,989 reads, respectively. Through annotation, a total of 110,073 and 15,795 OTUs of bacteria and archaea were obtained, and 61 bacterial phyla and 15 archaeal phyla were identified, of which the dominant phyla were Proteobacteria and Thaumarchaeota in deep-sea sediment. Therefore, our findings provided a biodiversity data of microbial communities in deep-sea sediment at global-scale and laid a foundation to further reveal the structures of microorganism communities in deep sea.

Effects of vibration training on balance stability in long jumpers / Efeitos do treinamento de vibração na estabilidade do equilíbrio em saltadores de salto em distância / Efectos del entrenamiento de vibración sobre la estabilidad del equilibrio en saltadores de longitud

ABSTRACT Introduction Vibration training refers to the introduction of vibration methods into the daily training of athletes for body stimulation. In this way, coaches improve athletes' strength, flexibility, and balance. Athletes can use vibration to stimulate neuromuscular activation. This allows more active units to be concentrated in the muscular contraction. In this way, the athlete's muscular strength can be increased. Objective This study aimed to analyze the relationship between vibration training and the balance ability of long jumpers. Methods This paper selects several long jumpers as research subjects. The effects of vibration exercises at different frequencies on athletes' stability are discussed, employing experimental comparison. In this context, statistical calculations are performed on the experimental results. Results There was no difference in balance ability between the vibration and general training groups before training (P>0.05). The athletes in the vibration training group showed more significant improvement in balance after a training period than the general training group. There were significant differences in the data between the two groups (P<0.01). Conclusion Vibration strength training can effectively improve the balance ability of jumpers. Athletes should increase the frequency of vibration training in their daily training. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução O treinamento vibratório refere-se à introdução de métodos de vibração no treinamento diário dos atletas para estimulação corporal. Desta forma, os treinadores melhoram a força, a flexibilidade e o equilíbrio dos atletas. Os atletas podem usar as vibrações para estimular a ativação neuromuscular. Isto permite que mais unidades ativas sejam concentradas na contração muscular. Desta forma, a força muscular do atleta pode ser aumentada. Objetivo Este estudo teve como objetivo analisar a relação entre o treinamento por vibração e a capacidade de equilíbrio dos saltadores de salto em distância. Métodos Este trabalho seleciona vários saltadores de salto em distância como objetos de pesquisa. Discute-se os efeitos dos exercícios de vibração em diferentes freqüências sobre a estabilidade do atleta, empregando a comparação experimental. Neste contexto, são efetuados cálculos estatísticos sobre os resultados experimentais. Resultados Não houve diferença na capacidade de equilíbrio entre o grupo de vibração e os grupos de treinamento geral antes do treinamento (P>0,05). Os atletas do grupo de treinamento de vibração mostraram uma melhora mais significativa no equilíbrio após um período de treinamento do que no grupo de treinamento geral. Houveram diferenças significativas nos dados entre os dois grupos (P<0,01). Conclusão O treinamento de força vibratória pode efetivamente melhorar a capacidade de equilíbrio dos saltadores. Os atletas devem aumentar a freqüência do treinamento por vibração em seu treinamento diário. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción El entrenamiento con vibraciones se refiere a la introducción de métodos de vibración en el entrenamiento diario de los deportistas para la estimulación del cuerpo. De este modo, los entrenadores mejoran la fuerza, la flexibilidad y el equilibrio de los deportistas. Los atletas pueden utilizar la vibración para estimular la activación neuromuscular. Esto permite concentrar más unidades activas en la contracción muscular. De este modo, se puede aumentar la fuerza muscular del deportista. Objetivo Este estudio tiene como objetivo analizar la relación entre el entrenamiento de vibración y la capacidad de equilibrio de los saltadores de longitud. Métodos En este trabajo se seleccionan varios saltadores de longitud como objeto de investigación. Se discuten los efectos de los ejercicios de vibración a diferentes frecuencias sobre la estabilidad del atleta, empleando la comparación experimental. En este contexto, se realizan cálculos estadísticos sobre los resultados experimentales. Resultados No hubo diferencias en la capacidad de equilibrio entre el grupo de vibración y los grupos de entrenamiento general antes del entrenamiento (P>0,05). Los atletas del grupo de entrenamiento con vibración mostraron una mejora significativa del equilibrio tras un periodo de entrenamiento que los del grupo de entrenamiento general. Hubo diferencias significativas en los datos entre los dos grupos (P<0,01). Conclusión El entrenamiento de fuerza con vibración puede mejorar eficazmente la capacidad de equilibrio de los saltadores. Los deportistas deben aumentar la frecuencia del entrenamiento con vibraciones en su entrenamiento diario. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

Safety and efficacy of thermal ablation for cervical metastatic lymph nodes in papillary thyroid carcinoma: A systematic review and meta-analysis.

Background: To evaluate the safety and efficacy of radiofrequency ablation (RFA), microwave ablation (MWA), and laser ablation (LA) for the treatment of cervical metastatic lymph nodes (CMLNs) of papillary thyroid carcinoma (PTC). Methods: The Pubmed, EMBASE, Web of Science, and Cochrane Library databases were searched for studies on the safety and efficacy of thermal ablations (RFA, MWA, and LA) for the treatment of CMLNs of PTC until March 30, 2022. A review of 334 potential papers identified 17 eligible papers including 312 patients. Fixed-effects model or random-effects model was used to evaluate the pooled proportions of volume reduction rate (VRR), complete disappearance, and recurrence, and pooled estimates of changes in the largest diameter, volume, and serum Tg after ablation. The pooled proportions of overall and major complications were calculated. Subgroup analysis based on treatment modalities. The heterogeneity among studies was analyzed by using Q statistics and inconsistency index I2 . MINORS scale was used to evaluate the quality of the studies. Results: 17 eligible studies were finally identified, including 312 patients and 559 CMLNs. The pooled proportions of VRR, complete disappearance and recurrence of CMLNs were 91.28% [95% confidence interval (CI): 86.60-95.97%], 67.9% [95% CI: 53.1-81.1%] and 7.8% [95%CI: 3.0-14.1%], respectively. The pooled estimates of changes in the largest diameter, volume and serum Tg were 8.12 mm [95%CI: 6.78-9.46 mm], 338.75 mm3 [95%CI: 206.85 -470.65 mm3] and 5.96 ng/ml [95%CI: 3.68-8.24 ng/ml], respectively. The pooled proportions of overall and major complications were 2.9% [95%CI: 0.3-7.1%] and 0.3% [95%CI: 0-1.9%], respectively. Significant between-study heterogeneity was observed for complete disappearance (P<0.01, I2 =88.6%), VRR (P<0.001, I2 =99.9%), recurrence (P=0.02, I2 =47.76%), overall complications (P<0.02, I2 =44.8%), and changes in the largest diameter (P < 0.001, I2 =82.6%), volume (P<0.001, I2 =97.0%), and serum Tg (P < 0.001, I2 =93.7%). Subgroup analysis showed heterogeneity of the VRR among the treatment modality (I2 range: 84.4-100%). The VRR of MWA was the highest (97.97%), followed by RFA (95.57%) and LA (84.46%) (P < 0.001). Conclusion: All thermal ablations were safe and effective for the treatment of CMLNs of PTC. However, each treatment had significant heterogeneity in VRR. Compared with RFA and MWA, LA was less effective in reducing the volume of CMLNs of PTC.

Evaluating the safety and efficacy of microwave ablation in treatment of cervical metastatic lymph nodes of papillary thyroid carcinoma compared to repeat surgery.

OBJECTIVE: To evaluate the safety and efficacy of microwave ablation (MWA) versus repeat surgery for treating metastatic lymph nodes (MLNs) in papillary thyroid carcinoma (PTC). METHODS: Between July 2017 and October 2020, 67 patients were enrolled in this retrospective study. 19 and 48 patients underwent MWA and repeat surgery, respectively. The primary and secondary endpoints were recurrence-free survival and complication rates, respectively. The largest diameter, volume and volume reduction ratio (VRR) were analyzed before and after MWA. The effects of different ablation powers on the largest diameter, volume and VRR were investigated. Pre and posttreatment variables (e.g., baseline characteristics, serum thyroglobulin [Tg] levels, hospitalization time, treatment costs, recurrence-free survival and complication rates) were compared between groups. RESULTS: The largest diameter and volume postablation at each follow-up were smaller than the preablation levels (p < 0.05), except at the 1-month follow-up (p > 0.05). The largest diameter, volume, and VRR among the different ablation powers were not significantly different (p > 0.05). The mean serum Tg levels and biochemical remission rates were not significantly different between the groups (p > 0.05). Compared to reoperation, MWA had a shorter hospitalization time and lower treatment cost (p < 0.001). Total and minor complications were higher in the reoperation group (p < 0.05), but major complications were comparable (p > 0.05). The recurrence-free survival rate between groups was not significantly different (p = 0.401). The 1- and 3-year recurrence-free survival rates were comparable between the groups. CONCLUSIONS: MWA may be a safe and effective alternative to repeat surgery for treating MLNs of PTC in select patients.

Strategies for improving the catalytic activity of metal-organic frameworks and derivatives in SR-AOPs: Facing emerging environmental pollutants.

As persulfate activator, Metal organic frameworks (MOFs) and derivatives are widely concerned in degradation of emerging environmental pollutants by advanced oxygen technology dominated by sulfate radical () (SR-AOPs). However, the poor stability and low catalytic efficiency limit the performance of MOFs, requiring multiple strategies to further enhance their catalytic activity. The aim of this paper is to improve the catalytic activity of MOFs and their derivatives by physical and chemical enhancement strategies. Physical enhancement strategies mainly refer to the activation strategies including thermal activation, microwave activation and photoactivation. However, the physical enhancement strategies need energy consumption and require high stability of MOFs. As a substitute, chemical enhancement strategies are more widely used and represented by optimization, modification, composites and derivatives. In addition, the identification of reactive oxygen species, active site and electron distribution are important for distinguishing radical and non-radical pathways. Finally, as a new wastewater treatment technology exploration of unknown areas in SR-AOPs could better promote the technology development.

Continued versus discontinued oxytocin after the active phase of labor: An updated systematic review and meta-analysis.

OBJECTIVE: To systematically assess the effect of discontinued vs continued oxytocin after active stage of labour is established. METHODS: Pubmed, Embase, and the Cochrane Library were systematically searched to 18 April 2021. The risk ratio or mean difference with corresponding 95% confidence interval were computed to investigate the effect of intervention or control on maternal and fetus outcomes. This review was registered in the International Prospective Register of Systematic Reviews: CRD42021249635. RESULTS: Discontinuing oxytocin when the active labour was established might decrease the risk of cesarean delivery [RR (95% CI): 0.84 (0.72-0.98), P = 0.02]. However, when we restricted our analysis to women who performed cesarean section after the active phase was reached, the difference was no longer significant [RR (95% CI): 0.82 (0.60-1.10), P = 0.19]. The incidence of uterine tachysystole [RR (95% CI): 0.36 (0.27-0.49)], postpartum hemorrhage [RR (95% CI): 0.78 (0.65-0.93)], and non-reassuring fetal heart rate [RR (95% CI): 0.66 (0.58-0.76)] were significantly lower in the oxytocin discontinuation group. We also found a possible decrease in the risk of chorioamnionitis in discontinued oxytocin group [RR (95% CI): 2.77 (1.02-5.08)]. An increased duration of active [MD (95% CI): 2.28 (2.86-41.71)] and second [MD (95% CI): 5.36 (3.18-7.54)] phase of labour was observed in discontinued oxytocin group, while the total delivery time was not significantly different [MD (95% CI): 20.17 (-24.92-65.26)]. CONCLUSION: After the active labor is reached, discontinuation of oxytocin could be considered a new recommendation for the improved maternal and fetal outcomes without delaying labour.

Laparoendoscopic single-site compared with conventional laparoscopic surgery for gynaecological acute abdomen in pregnant women.

OBJECTIVE: To estimate the safety and feasibility of laparoendoscopic single-site surgery (LESS) in pregnant patients with acute abdomen. METHODS: Baseline characteristics, surgical results, and obstetric and neonatal outcomes were retrospectively compared between single and multiport procedures in patients who underwent laparoscopic surgery during pregnancy between 2017 and 2021. RESULTS: Fifty-four pregnant patients were included: 26 who underwent LESS (salpingectomy, 11 cases/cystectomy, 15 cases) and 28 who underwent conventional laparoscopic surgeries (salpingectomy, 12 cases/cystectomy, 16 cases) during pregnancy. One patient in the single-port group required additional ports. No patients converted to laparotomy. In patients undergoing salpingectomy, the single-port group showed lower 8- and 24-h postoperative pain scores, shorter hospital stays, and lower Self-rating Anxiety Scale scores prior to discharge versus conventional laparoscopy. One patient experienced postoperative vaginal bleeding and a missed abortion during follow-up. In patients receiving cystectomy, 8- and 24-h pain scores, postoperative hospital stay, and anxiety scores were lower in the single-port versus multiport group. Other outcomes were comparable between the groups. CONCLUSION: The feasibility and efficacy of laparoscopic surgery during pregnancy is similar between single- or multiport routes, however, the single-port route may be associated with less postoperative pain, shorter hospital stay, and lower anxiety.

Analysis and evaluation of the efficacy of ultrasound-guided microwave ablation for papillary thyroid microcarcinoma.

OBJECTIVE: The choice of the most appropriate therapeutic approach for a diagnosed papillary thyroid microcarcinoma (PTMC) remains controversial. The present study aimed to evaluate the efficacy of microwave ablation (MWA) for unifocal PTMC with a diameter of ≤0.6 cm. METHODS: A total of 63 consecutive patients with PTMC treated with MWA were studied retrospectively. MWA was performed using the hydrodissection technique and multidimensional fixed-needle principle. We analyzed the absorption of the MWA area and evaluated the prognosis over a follow-up period of 24 months. In addition, 83 patients with PTMC who underwent surgery were selected. The operating room characteristics and procedural complications of the two groups were compared. RESULTS: In the MWA group, the volume of nodules (p < 0.05) decreased from 0.04 ± 0.03 cm3 to 0.0001 ± 0.0004 cm3 at the 24-month follow-up after MWA, and the volume reduction rate (p < 0.05) was 99.43 ± 1.58%. The incidence of temporary reactive hyperplastic lymphadenectasis was higher and that of other complications was lower in the MWA group than in the surgery group. One percent of the patients in the surgery group had recurrence or metastasis, but none were detected in the MWA group. The loss of thyroid tissue volume (p < 0.001), operating room time (p < 0.001), and the mean length of hospital stay (p < 0.001) were significantly lower in the MWA group than in the surgery group. CONCLUSION: Ultrasound-guided MWA is an effective treatment strategy for unifocal PTMC with a diameter of ≤0.6 cm.

Octadecylimidazolium ionic liquids-functionalized carbon dots and their precursor co-immobilized silica as hydrophobic chromatographic stationary phase with enhanced shape selectivity.

In this work, 1-vinyl-3-octadecylimidazolium bromide ionic liquids ([C18VIm]Br) and their derived carbon dots (ImC18CDs) were prepared, [C18VIm]Br and ImC18CDs were grafted on the silica to obtain Sil-ImC18 and Sil-ImC18CDs, respectively, and they were also co-grafted on silica which named Sil-ImC18/CDs. Compared with Sil-ImC18 and Sil-ImC18CDs columns, Sil-ImC18/CDs column exhibited enhanced selectivity for separation of tetracyclic/tricyclic polycyclic aromatic hydrocarbon (PAH) isomers, and butylbenzene isomers in reversed-phase liquid chromatography, which may be due to the synergistic effect between ImC18CDs and [C18VIm]Br, the π-π interaction between imidazolium and analytes, etc. Meanwhile, the retention behavior of Sil-ImC18/CDs was further evaluated and compared with the commercial C18 column using different classes of analytes, including standard test mixtures of Tanaka, Engelhardt, SRM869b, SRM870. The results demonstrated that co-grafted column exhibited superior separation performance. And this column was applied to determine the contents of calycosin-7-glucoside, ononin, calycosin and formononetin in the extract of Radix Astragali, which were found that the concentration was 0.25 mg mL-1, 0.15 mg mL-1, 0.13 mg mL-1 and 0.30 mg mL-1, respectively.

Risk factors for ovarian endometrioma recurrence following surgical excision: a systematic review and meta­analysis.

PURPOSE: Exploring potential risk factors for OMA recurrence, thereby contributing to the individual management of the disease and improving the patients' prognosis. METHODS: Data sources PubMed, Embase, the Cochrane Library, CNKI, and Wanfang data were searched systematically before October 2020. We computed the pooled odd ratios or the standard mean difference with their corresponding 95% confidence interval to investigate the impact of involved risk factors on endometrioma recurrence. RESULTS: The pooled findings of this meta-analysis demonstrated that endometrioma relapse was closely related to age at surgery [SMD (95% CI):  - 0.28 (- - 0.38 to  - 0.17), P < 0.00001], CA125 level [SMD (95% CI): 0.51 (0.14-0.88), P = 0.007], cyst size [SMD (95% CI): 0.35 (0.08-0.62), P = 0.01], dysmenorrhea [OR (95% CI): 1.47 (1.07-2.02), P = 0.02], endometriosis-related surgery history [OR (95% CI): 2.60 (1.84-3.67), P < 0.00001], pre-operative medication [OR (95% CI): 2.13 (1.41-3.22), P = 0.0003], rASRM score [SMD (95% CI): 0.33 (0.20-0.46), P < 0.00001]. Furthermore, post-operative pregnancy was indicated a protective factor for preventing the OMA recurrence after surgery [OR (95% CI): 0.22 (0.09-0.56), P = 0.001] CONCLUSION: Age at surgery, CA125 level, cyst size, dysmenorrhea, endometriosis-related surgery history, pre-operative medication, rASRM score were risk factors for endometrioma relapse. In addition, post-operative pregnancy was a protective factor for preventing recurrence after surgery. However, the effect of bilateral involvement, combination with adenomyosis, or post-operative medication on endometrioma relapse need further investigations.

Effect of endometrioma and its surgical excision on fertility (Review).

Endometrioma is the cystic lesion of ovaries originating from endometrial glands and stroma; it is identified in 17-44% of patients with endometriosis. Numerous existing studies have reported the association between endometrioma and infertility. However, an absolute cause-effect association requires further confirmation. Available evidence has suggested that ovarian reserve may be impaired by spatial occupation influences, local reaction or both, affecting the reproductive health of females. Given the increased focus on the pathophysiological mechanisms of endometrioma, surgical excision has commonly been considered to avoid further ovarian damage. However, the potential adverse effect of this surgery on the ovarian reserve has recently become a focal point. Whether or not surgical excision can facilitate subsequent conception in young females planning to be pregnant is controversial. As shown in the present review on the effects of endometrioma and its removal in females requiring assisted reproductive technology, prior surgery for endometrioma may not improve assisted fertility results and may further decrease the number of oocytes retrieved in the affected females. Subsequent studies are needed to ascertain the optimal management of infertility in the setting of endometriomas.

Preparation of silver-nanoparticle-loaded magnetic biochar/poly(dopamine) composite as catalyst for reduction of organic dyes.

In the present study, Ag nanoparticles loaded on polydopamine coated magnetic biochar (MC-PDA-Ag) catalyst was prepared by in-situ reduction approach. The morphology, composition, and structure of MC-PDA-Ag were characterized by the transmission electron microscopy (TEM), the scanning electron microscope (SEM), X-ray diffractometer (XRD), X-ray photoelectron spectroscopy (XPS), and vibrating sample magnetometer (VSM). Catalytic reduction tests confirmed that MC-PDA-Ag catalyst showed excellent catalytic performance on the model dyes (MB, RhB, and MO) in the presence of NaBH4. The reduction efficiency of MB exceeded 90% by MC-PDA-Ag even under different initial pH (3-11) and different NaNO3 concentrations (0-0.5 M) within 5 min, indicating that the MC-PDA-Ag catalyst has potential strong universal adaptability in dye wastewater treatment. Furthermore, no significant decrease in catalytic ability was observed after 5 recycles, and the catalyst could be separated by an external magnet, indicating that this as-prepared catalyst exhibited high reusability and easy separability. These results suggest that MC-PDA-Ag composite catalyst can be used as an efficient catalyst for the reduction of organic dyes, and shows great potential application in wastewater treatment.

Metal Organic Frameworks as Robust Host of Palladium Nanoparticles in Heterogeneous Catalysis: Synthesis, Application, and Prospect.

Metal organic frameworks (MOFs) are one set of the most excellent supports for Pd nanoparticles (NPs). MOFs as the host mainly have the following advantages: (i) they provide size limits for highly dispersed Pd NPs; (ii) fixing Pd NPs is beneficial for separation and reuse, avoiding the loss of expensive metals; (iii) the MOFs skeleton is diversified and functionalized, which is beneficial to enhancing the interaction with Pd NPs and prolonging the service life of the catalyst. This review discusses the synthesis strategy of Pd@MOF, which provides guidance for the synthesis of similar materials. After that, the research advance of Pd@MOF in heterogeneous catalysis is comprehensively summarized, including C-C coupling reaction, benzyl alcohol oxidation reaction, simple olefin hydrogenation reaction, nitroaromatic compound reduction, tandem reaction, and the photocatalysis, with the emphasis in providing a comparison with the performance of other alternative Pd-containing catalysts. In the final section, this review presents the current challenges and which are the next goals in this field.

Understanding the influence of carbon nanomaterials on microbial communities.

Carbon nanomaterials (CNMs) are widely used because of their unique advantages in recent years. At the same time, the influence of CNMs on the environment is becoming increasingly prominent. This review mainly introduces the research progress in the effects of fullerenes, multi-walled carbon nanotubes (MWCNTs), single-walled carbon nanotubes (SWCNTs) and graphene on microorganisms and their toxicity mechanisms. On this basis, we have analyzed beneficial and adverse effects of fullerenes, graphene, MWCNTs and SWCNTs to microorganisms, and discussed the similarities of the toxicity mechanisms of different CNMs on microorganisms. This review helps provide ideas on how to protect microorganisms from the impacts of carbon nanomaterials, and it will be conductive to providing a strong theoretical basis for better application of carbon nanomaterials.

p53-independent role of MYC mutant T58A in the proliferation and apoptosis of breast cancer cells.

Myc proto-oncogene (MYC) is an oncoprotein that promotes proliferation and apoptosis. MYC mutations frequently disrupt the apoptotic processes during tumorigenesis. In the present study, the effects of the MYC point mutation T58A on the progression of a cellular tumor antigen p53 (p53)-/- human breast cancer cell line was analyzed, and the mechanism of p53-independent MYC-induced apoptosis was investigated. HCC1937 cells were transfected with mutant (T58A) or wild-type (WT) MYC using lentiviral vectors. The proliferation of transfected cells was evaluated by colony formation and MTT assays, and apoptosis was analyzed by flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assays. WT MYC was transfected into HCC1937 cells exhibiting p14/p21 silencing through lentivirus-mediated RNA interference. The expression levels of Bim were detected by reverse transcription-quantitative polymerase chain reaction and western blot analyses. Mutant MYC proteins retained the ability to stimulate the proliferation of HCC1937 cells, although they were defective at promoting apoptosis due to a failure to induce the Bcl-2 homology 3 domain-only protein Bim. When p14 was silenced, the effects of mutant MYC on proliferation and apoptosis were weakened. When p21 was silenced, the effects of mutant MYC were strengthened. Breast cancer-derived T58A MYC mutations are unable to activate Bim due to their failure to regulate p14/p21. It was concluded that mutant MYC was more effective compared with WT MYC at promoting the progression of breast cancer.